Quantitative determination of phyllanthin and hypophyllanthin in Phyllanthus species by high‐performance thin layer chromatography
Phytochemical Analysis: An International Journal of Plant Chemical …, 2006•Wiley Online Library
A simple, precise and rapid high‐performance thin‐layer chromatographic method has been
developed for the estimation of phyllanthin (1) and hypophyllanthin (2), the important lignans
of Phyllanthus species, especially Phyllanthus amarus. Separation of 1 and 2 was carried
out on silica gel 60 F254 layers eluted with hexane: acetone: ethyl acetate (74: 12: 8), and
the analytes were visualised through colour development with vanillin in concentrated
sulphuric acid and ethanol. Scanning and quantification of spots was performed at 580 nm …
developed for the estimation of phyllanthin (1) and hypophyllanthin (2), the important lignans
of Phyllanthus species, especially Phyllanthus amarus. Separation of 1 and 2 was carried
out on silica gel 60 F254 layers eluted with hexane: acetone: ethyl acetate (74: 12: 8), and
the analytes were visualised through colour development with vanillin in concentrated
sulphuric acid and ethanol. Scanning and quantification of spots was performed at 580 nm …
Abstract
A simple, precise and rapid high‐performance thin‐layer chromatographic method has been developed for the estimation of phyllanthin (1) and hypophyllanthin (2), the important lignans of Phyllanthus species, especially Phyllanthus amarus. Separation of 1 and 2 was carried out on silica gel 60 F254 layers eluted with hexane:acetone:ethyl acetate (74:12:8), and the analytes were visualised through colour development with vanillin in concentrated sulphuric acid and ethanol. Scanning and quantification of spots was performed at 580 nm. Recoveries of 1 and 2 were 98.7 and 97.3%, respectively. The method was validated and the peak purities and limits of detection and quantification were determined. Copyright © 2006 John Wiley & Sons, Ltd.
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