Rhinosinusitis is mostly affected by viral infections and allergy and resolves without antibiotics usage. However, antibiotics are still frequently used as drug of choice for rhinosinusitis. If conservative treatments failed, surgical procedures had to be done. Neurogenic inflammation is involved in rhinosinusitis. Possibly because rhinosinusitisinduced chronic gingivitis successfully relieved by the “assisted drainage therapy”(ADT). This new periodontal therapy consists of scaling and root planing followed by subgingival massage. However, the mechanism had not been clearly verified. To verify the mechanism of allergic rhinosinusitis symptoms relief by ADT. Randomized control time series design experimental study was conducted in two groups of Wistar rats. Allergic sensitization was performed by injections and inhalation of ovalbumin (OVA). Gingivitis was induced by synthetic Porphyromonas gingivalis lipopolysaccharide (PgLPS1435/1450). One group was subjected to ADT before inhalation and another groups without ADT. Immunohistochemistry for biomarkers of allergic reaction (LTC4 and ECP) and neurogenic inflammation (CGRP and VIP) performed. After OVA inhalation, allergic reaction and neurogenic inflammation biomarkers had significantly lower in rats subjected to ADT before inhalation than without ADT (p= 0.001). ADT significantly decreased nasal allergic reaction and neurogenic inflammation biomarkers, therefore could be proposed as an adjunct therapy of allergic rhinosinusitis.