Rapid and visual detection of Leptospira in urine by LigB-LAMP assay with pre-addition of dye
Leptospirosis is considered to be the most widespread zoonotic disease caused by
pathogenic species of Leptospira. The present study reports a novel set of primers targeting
LigB gene for visual detection of pathogenic Leptospira in urine samples through Loop-
mediated isothermal amplification (LAMP). The results were recorded by using Hydroxyl
napthol blue (HNB), SYBR GREEN I and calcein. Analytical sensitivity of LAMP was as few
as 10 leptospiral organisms in spiked urine samples from cattle and dog. LigB gene based …
pathogenic species of Leptospira. The present study reports a novel set of primers targeting
LigB gene for visual detection of pathogenic Leptospira in urine samples through Loop-
mediated isothermal amplification (LAMP). The results were recorded by using Hydroxyl
napthol blue (HNB), SYBR GREEN I and calcein. Analytical sensitivity of LAMP was as few
as 10 leptospiral organisms in spiked urine samples from cattle and dog. LigB gene based …
以上显示的是最相近的搜索结果。 查看全部搜索结果