Rapid determination of nitrite by reversed-phase high-performance liquid chromatography with fluorescence detection
H Li, CJ Meininger, G Wu - … of Chromatography B: Biomedical Sciences and …, 2000 - Elsevier
Measurement of nitrite and nitrate, the stable oxidation products of nitric oxide (NO),
provides a useful tool to study NO synthesis in vivo and in cell cultures. A simple and rapid
fluorometric HPLC method was developed for determination of nitrite through its
derivatization with 2, 3-diaminonaphthalene (DAN). Nitrite, in standard solution, cell culture
medium, or biological samples, readily reacted with DAN under acidic conditions to yield the
highly fluorescent 2, 3-naphthotriazole (NAT). For analysis of nitrate, it was converted to …
provides a useful tool to study NO synthesis in vivo and in cell cultures. A simple and rapid
fluorometric HPLC method was developed for determination of nitrite through its
derivatization with 2, 3-diaminonaphthalene (DAN). Nitrite, in standard solution, cell culture
medium, or biological samples, readily reacted with DAN under acidic conditions to yield the
highly fluorescent 2, 3-naphthotriazole (NAT). For analysis of nitrate, it was converted to …
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