Ras, Rap, and Rac Small GTP-binding Proteins Are Targets for Clostridium sordellii Lethal Toxin Glucosylation (∗)
MR Popoff, E Chaves-Olarte, E Lemichez… - Journal of Biological …, 1996 - ASBMB
Lethal toxin (LT) from Clostridium sordellii is one of the high molecular mass clostridial
cytotoxins. On cultured cells, it causes a rounding of cell bodies and a disruption of actin
stress fibers. We demonstrate that LT is a glucosyltransferase that uses UDP-Glc as a
cofactor to covalently modify 21-kDa proteins both in vitro and in vivo. LT glucosylates Ras,
Rap, and Rac. In Ras, threonine at position 35 was identified as the target amino acid
glucosylated by LT. Other related members of the Ras GTPase superfamily, including RhoA …
cytotoxins. On cultured cells, it causes a rounding of cell bodies and a disruption of actin
stress fibers. We demonstrate that LT is a glucosyltransferase that uses UDP-Glc as a
cofactor to covalently modify 21-kDa proteins both in vitro and in vivo. LT glucosylates Ras,
Rap, and Rac. In Ras, threonine at position 35 was identified as the target amino acid
glucosylated by LT. Other related members of the Ras GTPase superfamily, including RhoA …
以上显示的是最相近的搜索结果。 查看全部搜索结果