Rate theories and puzzles of hemeprotein kinetics

H Frauenfelder, PG Wolynes - Science, 1985 - science.org
H Frauenfelder, PG Wolynes
Science, 1985science.org
The binding of dioxygen and carbon monoxide to heme proteins such as myoglobin and
hemoglobin has been studied with flash photolysis. At temperatures below 200 K, binding
occurs from within the heme pocket and, contrary to expectation, with nearly equal rates for
both ligands. This observation has led to a reexamination of the theory of the association
reaction taking into account friction, protein structure, and the nature of electronic transitions.
The rate coefficients for the limiting cases of large and small friction are found with simple …
The binding of dioxygen and carbon monoxide to heme proteins such as myoglobin and hemoglobin has been studied with flash photolysis. At temperatures below 200 K, binding occurs from within the heme pocket and, contrary to expectation, with nearly equal rates for both ligands. This observation has led to a reexamination of the theory of the association reaction taking into account friction, protein structure, and the nature of electronic transitions. The rate coefficients for the limiting cases of large and small friction are found with simple arguments that use characteristic lengths and times. The arguments indicate how transition state theory as well as calculations based on nonadiabatic perturbation theory, which is called the Golden Rule, may fail. For ligand-binding reactions the data suggest the existence of intermediate states not directly observed so far. The general considerations may also apply to other biomolecular processes such as electron transport.
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