In this paper, an analytical basis of ray tracing for optical biometry of media by ellipsoidal reflectors was developed. A ray-tracing algorithm was created, which involves determining the point of interaction of the incident beam with the ellipsoidal reflector, calculating the direction of the reflected beam, and determining the intersection point of the reflected ray with the second ellipsoid focal plane, taking into account the statistical weight of photons. For imaging in the optical biometry of media by ellipsoidal reflectors, the results of a real experiment and a Monte Carlo simulation for chicken muscle tissue samples of different thicknesses for the wavelength of 632.8 nm are used.