However, it is challenging to analyze large-scale imaging data in real time, preventing
closed-loop investigation of neural circuitry. Here we develop a real-time analysis system
with a field programmable gate array–graphics processing unit design for an up to 500-
megabyte-per-second image stream. Adapted to whole-brain imaging of awake larval
zebrafish, the system timely extracts activity from up to 100,000 neurons and enables closed …