… PCR multiplexing by Tm, a powerful tool with a multiThe ability to multiplex PCR by probe …
emisture (Tm) greatly expands the power of real-time analysis. Simple sion spectra. When both …

… We present an SYBR-Green-based, real-time multiplex PCR assay for rapid identification
and differentiation of scrub typhus group, typhus group and spotted fever group rickettsiae …

… multiplex PCR assay for HRSV were 94.5 and 98.9% compared to 81.6 and 94.7% for the
antigenic test. In conclusion, our multiplex real-time PCR assay … real-time multiplex PCR assay …

… The aim of this study was to develop and evaluate two multiplex real-time PCR assays for …
Furthermore, all 249 positive control isolates were correctly detected with our assay, …

… In this study, a conventional multiplex PCR and a SYBR Green based real time multiplex
assays were carried out in two independent experiments to detect six commonly occurring …

… , while the multiplex is faster but may cause problems in loss of sensitivity. We herewith
report the establishment and validation of three real-time multiplex PCR assays that are highly …

… real-time multiplex PCR assay and traditional cultural method showed 100% inclusivity and
100% exclusivity on all strains tested. The real-time multiplex PCR assay … the real-time PCR …

… Here, we describe a real-time PCR assay for confirmation of presumptive Brucella isolates.
The assay was designed in a multiplex format that will allow the rapid identification of …

… ) citing success with real-time multiplex PCR were compared and … multiplex PCR analysis
and found that their tests maintained high sensitivity and specificity during validation testing…

… Detection limit of the real-time, multiplexed PCR assay in feces. The sensitivity of the PCR
assay … coli (B) and analyzed using the real-time multiplex PCR assay. The mean and standard …