Reduction of false-negative papillary thyroid carcinomas by the routine analysis of BRAFT1799A mutation on fine-needle aspiration biopsy specimens: a prospective …
M Cañadas-Garre, P Becerra-Massare… - Annals of …, 2012 - journals.lww.com
M Cañadas-Garre, P Becerra-Massare, ML de la Torre-Casares, J Villar-del Moral…
Annals of surgery, 2012•journals.lww.comObjectives: To evaluate prospectively the usefulness of the routine determination of BRAF
T1799A mutation on thyroid fine-needle aspiration biopsy (FNAB) to detect cytopathology
false negative papillary thyroid carcinomas (PTC) and, therefore, as a tool to improve the
sensitivity of the preoperative cytopathological diagnosis of PTC in thyroid nodules.
Background: FNAB is the most reliable diagnostic test to discriminate between malignant
and benign thyroid nodules, but nondiagnostic results remain a clinical management …
T1799A mutation on thyroid fine-needle aspiration biopsy (FNAB) to detect cytopathology
false negative papillary thyroid carcinomas (PTC) and, therefore, as a tool to improve the
sensitivity of the preoperative cytopathological diagnosis of PTC in thyroid nodules.
Background: FNAB is the most reliable diagnostic test to discriminate between malignant
and benign thyroid nodules, but nondiagnostic results remain a clinical management …
Objectives:
To evaluate prospectively the usefulness of the routine determination of BRAF T1799A mutation on thyroid fine-needle aspiration biopsy (FNAB) to detect cytopathology false negative papillary thyroid carcinomas (PTC) and, therefore, as a tool to improve the sensitivity of the preoperative cytopathological diagnosis of PTC in thyroid nodules.
Background:
FNAB is the most reliable diagnostic test to discriminate between malignant and benign thyroid nodules, but nondiagnostic results remain a clinical management dilemma. BRAF T1799A mutation is the most prevalent genetic alteration in thyroid cancers and is specific for PTC, characteristics that make it the most potentially helpful genetic tool to improve the diagnostic accuracy of FNAB.
Methods:
An exhaustive recruitment of all patients subjected to thyroid FNAB in our institution during 4 years was performed. BRAF T1799A mutation was determined on thyroid FNAB specimens by PCR and restriction fragment length polymorphism, plus direct sequencing in positive samples.
Results:
BRAF T1799A mutation on FNAB detected 47.2%(17/36) of PTC cases. It confirmed preoperatively 45.5%(5/11) of the PTC cases in the indeterminate category and decreased the rate of cytopathology false-negatives in 33.3%(6/18), improving the combined (BRAF T1799A mutation+ cytopathological analysis) sensitivity of the detection of PTC on FNAB in 16.7%.
Conclusions:
BRAF T1799A mutation improves the diagnosis of PTC on FNAB, mainly because of the detection of cytopathology false-negatives, and it can be helpful in the routine analysis of thyroid nodules, especially in clinical settings with moderate sensitivity to detect PTC on FNAB.
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