Drug resistance presents a challenge to the treatment of cancer patients. Many studies have
focused on cell-autonomous mechanisms of drug resistance. By contrast, we proposed that
the tumour micro-environment confers innate resistance to therapy. Here we developed a co-
culture system to systematically assay the ability of 23 stromal cell types to influence the
innate resistance of 45 cancer cell lines to 35 anticancer drugs. We found that stroma-
mediated resistance is common, particularly to targeted agents. We characterized further the …

The Reproducibility Project: Cancer Biology seeks to address growing concerns about
reproducibility in scientific research by conducting replications of 50 papers in the field of
cancer biology published between 2010 and 2012. This Registered Report describes the
proposed replication plan of key experiments from 'Tumour micro-environment elicits innate
resistance to RAF inhibitors through HGF secretion'by Straussman and colleagues,
published in Nature in 2012. The key experiments being replicated in this study are from …