Simultaneous removal of nutrients (ammonium and phosphate) and COD was investigated by the co-culture consortium of microalga Chlorella vulgaris and bacterium Pseudomonas putida. The co-culture system showed higher removal of both nutrients and COD than the each axenic culture, indicating that nutrients uptake capability of C. vulgaris was enhanced in the presence of P. putida. The best performance in the removal of nitrogen, phosphorus, and COD was obtained through the co-culture with suspended P. putida and immobilized C. vulgaris, demonstrating that the employment of immobilization of one species is more synergistic than suspended co-culture system in nutrients removal from wastewater.