Nanotechnology are fast advancing and currently became more effective than the conventional technologies used in water treatment that offers safe opportunities for using unconventional water supply sources. Fungi are more versatile in growth and metal tolerance in contrast to bacterial population. This work aims to demonstrate the extracellular synthesis of silver nanoparticle by using two filamentous fungi Penciillium Citreonigum Dierck and Scopulaniopsos brumptii Salvanet-Duval isolated from Lake Burullus, examine the biosynthesized nano-silver particles by UV–vis spectroscopy, transmission electron microscopy (TEM). The functional group of protein molecules surrounding AgNPs was identified using Fourier transform infrared (FTIR) analysis. Check the antibacterial activity of biosynthesized silver nanoparticles at two concentrations (550.7 and 676.9 mg/l) and interact it with bacteria for different durations (15, 60 and 120 min). Polyurethane foam was used as silver carrier and nano-silver solution for the removal of pathogenic bacteria in polluted water. The synthesized AgNPs showed an excellent antibacterial property on gram positive and gram negative bacterial strains.