Purpose The purpose of this study was to characterize clinician-scientists in ophthalmology and identify factors associated with successful research funding, income, and career satisfaction. Design Cross-sectional study. Methods A survey was conducted of clinician-scientists in ophthalmology at US academic institutions between April 17, 2019, and May 19, 2019. Collected information including 1) demographic data; 2) amount, type, and source of startup funding; first extramural grant; and first R01-equivalent independent grant; 3) starting and current salaries; and 4) Likert-scale measurements of career satisfaction were analyzed using multivariate regression. Results Ninety-eight clinician-scientists in ophthalmology were surveyed across different ages (mean: 48±11 years), research categories, institutional types, geographic regions, and academic ranks. Median startup funding ranged from $50-99k, and median starting salaries ranged from $150-199k. A majority of investigators (67%) received their first extramural award from the National Eye Institute, mainly through K-award mechanisms (82%). The median time to receiving their first independent grant was 8 years, mainly through an R01 award (70%). Greater institutional startup support (P=. 027) and earlier extramural grant success (P=. 022) were associated with earlier independent funding. Male investigators (P=. 001) and MD degreed participants (P=. 008) were associated with higher current salaries but not starting salaries. Overall career satisfaction increased with career duration (P=. 011) but not with earlier independent funding (P=. 746) or higher income (P=. 300). Conclusions Success in research funding by clinician-scientists in ophthalmology may be linked to institutional support and earlier acquisition of extramural grants but does not impact academic salaries. Nevertheless, career satisfaction among clinician-scientists improves with time, which is not necessarily influenced by research or financial success.