Cadmium is one of the most toxic heavy metals and potential for human exposure has generally increased with increase in industrial usage of this element. The purpose of the present study was to determine anti-oxidative role of vitamin C against cadmium chloride induced oxidative stress on rat testis. Adult male rats (n= 6/group) were divided into five groups, one control (Gr. I-0.9% Saline treated) & two untreated experimental & two pretreated experimental groups. The untreated groups were injected with single dose of 0.5 & 1 mg/kg bw cadmium chloride (Gr. II &Gr. III) intraperitoneally. Vitamin C (30mg/kg bw, ip) was orally administered for 30 days prior to the exposure to 0.5 and 1mg/kg bw (Gr, IIa &Gr. IIIA) of cadmium chloride. In all the groups, rats were sacrificed 15 days after the final cadmium chloride or saline administration and the changes in the testicular weight and testicular level of Melonaldehyde, glutathione & superoxide dismutase were studied. Exposure to cadmium chloride lead to significant decrease in the testicular weight& level of GSH & SOD and increase in the level of testicular MDA compared to normal control. Pretreatment with vitamin C (30mg/kg bw) significantly prevented the increase in MDA level of the testis & ameliorated the fall in GSH & SOD as well as testicular weight compared to 0.5 mg/kg bw cadmium chloride group. But pretreatment with vitamin C did not show any beneficial effect with 1mg/kg bw cadmium treated group. The study reports the antioxidative role of vitamin C in ameliorating lower doses of cadmium chloride induced testicular damage.