[PDF][PDF] Schwann-like cell differentiation from rat bone marrow stem cells
IR Kashani, Z Golipoor, M Akbari… - Archives of Medical …, 2011 - termedia.pl
Archives of Medical Science, 2011•termedia.pl
Results: Immunocytochemistry staining and RT-PCR analysis revealed that the induced
BMSCs exhibited Schwann cell-specific markers such as S-100, P75 and glial fibrillary
acidic protein (GFAP) at the 14 th day of differentiation. MTT assay and flow cytometry
revealed that of the total BMSCs in the differentiation medium, 40% to 50% of the cells died
by apoptosis, but the remaining cell population remained strongly attached to the substrate
and differentiated. Conclusions: These findings indicated that BMSCs could differentiate into …
BMSCs exhibited Schwann cell-specific markers such as S-100, P75 and glial fibrillary
acidic protein (GFAP) at the 14 th day of differentiation. MTT assay and flow cytometry
revealed that of the total BMSCs in the differentiation medium, 40% to 50% of the cells died
by apoptosis, but the remaining cell population remained strongly attached to the substrate
and differentiated. Conclusions: These findings indicated that BMSCs could differentiate into …
Results: Immunocytochemistry staining and RT-PCR analysis revealed that the induced BMSCs exhibited Schwann cell-specific markers such as S-100, P75 and glial fibrillary acidic protein (GFAP) at the 14 th day of differentiation. MTT assay and flow cytometry revealed that of the total BMSCs in the differentiation medium, 40% to 50% of the cells died by apoptosis, but the remaining cell population remained strongly attached to the substrate and differentiated.
Conclusions: These findings indicated that BMSCs could differentiate into Schwann-like cells. As a side effect of differentiation an increased cell death rate was noted and our findings indicate that the principle mode of cell death is by apoptosis.
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