Heavy metal pollution poses a serious threat to all life forms in the environment due to its harmful effects and persistent nature. At low quantities, these metals are highly reactive and can accumulate in the food web, posing serious public health risks. Traditional physical and chemical methods of remediation are inefficient and produce enormous amounts of chemical waste. Over the years, bioremediation of toxic metals has attracted a lot of attention. In addition, their environmental friendliness and financial effectiveness make them a viable option for heavy metal contamination cleanup. Fungal isolates from rhizospheric soils of different locations of Raiganj, Uttar Dinajpur were collected, identified, and screened to assess their resistance to Ni, Cd and Co. Six fungal isolates were selected to study their tolerance to heavy metals. Results showed that Trichoderma aureoviride TaN16 was observed as the most tolerant fungal species capable of growing at elevated levels of Ni, Cd and Co. Trichoderma yunnanense TaN17 was also found with good potential for heavy metal tolerance. The exploration of the resistance mechanism of these fungi against the heavy metals can be helpful to know the bioremediation properties of them in future.