Habitat preference of juvenile shrimps as an indicator to determine of main shrimp nursery ground. Study on distribution and habitat preference of juvenile penaeid shrimps in Kubu Raya waters, West Kalimantan were carried out on June, September, November (2012) and April, July, October (2013). Sampling was conducted with a sweep area method using a mini beam trawl. Distribution pattern and habitat preference of juvenile shrimps were analyzed by constancy and fidelity index. Identification of juvenile penaeid shrimp consisted of 3 genera and 12 species with the highest of total abundance from genus of Metapenaeus (70.5%), followed by Parapenaeopsis (29.3%) and Fenneropenaeus (0.2%). Juvenile shrimp for those genera have different distribution patterns in certain habitats because of different life cycle pattern. The habitat preference of indian white shrimp juvenile (F. indicus) in the waters of Tanjung Bunga Dalam have very high category. Meanwhile, in the waters of Tanjung Intan and Pulau Dabung has medium category. The main nursery ground of F. indicus were estuaries with high enough of mangrove density and most affected by freshwater discharge with the dominant substrate type is sand as sandly mud and mix with litters. The habitat preference of Juvenile of fine shrimp (M. elegans) and thin shrimp (P. gracillima) have medium category in the waters of Tanjung Tempurung. The main nursery ground character needs estuaries that have a lot of freshwater and saltwater mass input depending on the tides with substrate type are mud, sand and mix with litters.