The dopamine-imprinted conducting polymer film of 5-amino 8-hydroxy quinoline (AHQ) was electrodeposited on reduced graphene oxide (rGO)-modified glassy carbon (GC) electrode and was applied as a molecular recognition element for the selective determination of dopamine. The molecularly imprinted polymer (MIP)-modified electrode showed an excellent affinity towards dopamine due to the presence of imprinted site through hydrogen bonding interaction between dopamine and poly (AHQ) membrane. The molecular recognition ability of MIP-modified electrode was analyzed by cyclic voltammetric and differential pulse voltammetric techniques. The most stable geometry of the template–monomer complex in the pre-polymerization mixture was calculated by computational approaches. The rGO modification augmented both surface area and electron transfer kinetics of the bare electrode. The GC/rGO/MIP electrode possessed 2.83 fold current enhancements when compared to GC/MIP electrode, indicating the improvement in sensitivity due to rGO modification. The limit of detection and sensitivity of GC/rGO/MIP electrode was observed to be 32.7 nM and 13.3 AM⁻¹ cm⁻², respectively. The imprinting methodology provided an exceptional selectivity towards the detection of dopamine even in the presence of high concentration of possible physiological interferents. Moreover, the fabricated electrode was successfully employed for the detection of dopamine in human blood plasma samples proving the effectiveness of the sensor for the sensitive detection of dopamine from real samples.