Self-protection mechanism in D-cycloserine-producing Streptomyces lavendulae: gene cloning, characterization, and kinetics of its alanine racemase and D-alanyl-D …
M Noda, Y Kawahara, A Ichikawa, Y Matoba… - Journal of Biological …, 2004 - ASBMB
An antibiotic, d-cycloserine (DCS), inhibits the catalytic activities of alanine racemase (ALR)
and d-alanyl-d-alanine ligase (DDL), which are necessary for the biosynthesis of the
bacterial cell wall. In this study, we cloned both genes encoding ALR and DDL, designated
alrS and ddlS, respectively, from DCS-producing Streptomyces lavendulae ATCC25233.
Each gene product was purified to homogeneity and characterized. Escherichia coli,
transformed with a pET vector carrying alrS or ddlS, displays higher resistance to DCS than …
and d-alanyl-d-alanine ligase (DDL), which are necessary for the biosynthesis of the
bacterial cell wall. In this study, we cloned both genes encoding ALR and DDL, designated
alrS and ddlS, respectively, from DCS-producing Streptomyces lavendulae ATCC25233.
Each gene product was purified to homogeneity and characterized. Escherichia coli,
transformed with a pET vector carrying alrS or ddlS, displays higher resistance to DCS than …
以上显示的是最相近的搜索结果。 查看全部搜索结果