Seminal plasma proteome of electroejaculated Bos indicus bulls

JPA Rego, JM Crisp, AA Moura, AS Nouwens… - Animal Reproduction …, 2014 - Elsevier
The present study describes the seminal plasma proteome of Bos indicus bulls. Fifty-six, 24-
month old Australian Brahman sires were evaluated and subjected to electroejaculation.
Seminal plasma proteins were separated by 2-D SDS-PAGE and identified by mass
spectrometry. The percentage of progressively motile and morphologically normal sperm of
the bulls were 70.4±2.3 and 64±3.2%, respectively. A total of 108 spots were identified in the
2-D maps, corresponding to 46 proteins. Binder of sperm proteins accounted for 55.8% of all …
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