Sequence Specific Modeling of E. coli Cell-Free Protein Synthesis
M Vilkhovoy, N Horvath, CH Shih… - ACS synthetic …, 2018 - ACS Publications
Cell-free protein synthesis (CFPS) is a widely used research tool in systems and synthetic
biology. However, if CFPS is to become a mainstream technology for applications such as
point of care manufacturing, we must understand the performance limits and costs of these
systems. Toward this question, we used sequence specific constraint based modeling to
evaluate the performance of E. coli cell-free protein synthesis. A core E. coli metabolic
network, describing glycolysis, the pentose phosphate pathway, energy metabolism, amino …
biology. However, if CFPS is to become a mainstream technology for applications such as
point of care manufacturing, we must understand the performance limits and costs of these
systems. Toward this question, we used sequence specific constraint based modeling to
evaluate the performance of E. coli cell-free protein synthesis. A core E. coli metabolic
network, describing glycolysis, the pentose phosphate pathway, energy metabolism, amino …
Sequence Specific Modeling ofE. coliCell-Free Protein Synthesis
Cell-free protein synthesis (CFPS) is a widely used research tool in systems and synthetic
biology. However, if CFPS is to become a mainstream technology for applications such as
point of care manufacturing, we must understand the performance limits and costs of these
systems. Toward this question, we used sequence specific constraint based modeling to
evaluate the performance of E. coli cell-free protein synthesis. A core E. coli metabolic
network, describing glycolysis, the pentose phosphate pathway, energy metabolism, amino …
biology. However, if CFPS is to become a mainstream technology for applications such as
point of care manufacturing, we must understand the performance limits and costs of these
systems. Toward this question, we used sequence specific constraint based modeling to
evaluate the performance of E. coli cell-free protein synthesis. A core E. coli metabolic
network, describing glycolysis, the pentose phosphate pathway, energy metabolism, amino …
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