Brucellosis, is an important zoonotic disease after rabies, causes significant reproductive losses in sexually mature animals and is a major barrier for the trade (Lopes et al., 2010; Abubakar et al., 2012). Although, brucellosis has been controlled or eradicated in many developed countries; yet it still remains an uncontrolled problem in regions of high endemicity such as the Mediterranean, Middle East, Africa, Central and Latin America, Eastern Europe, Caribbean and parts of Asia (Maurin and Maurin, 2005). The disease burden is more profound in the developing countries due to lack of effective public health measures, domestic animal health programs and appropriate diagnostic facilities. As no characteristic constellation of symptoms and signs exists, the diagnosis is usually missed (Gul and Khan, 2007). From public health view point, brucellosis is one of the world’s major zoonotic problems, categorized as class B bioterrorist agent and accounting for the annual occurrence of more than 500,000 cases (Seleem et al., 2010). This disease is considered to be an occupational disease that mainly affects slaughter-house workers, butchers, livestock producers, shepherds, farmers, veterinarians, and laboratory technicians (Behzadi and Mogheiseh, 2011). This disease has been imported from brucellosis-endemic countries into non-endemic areas due to increase in business and leisure travel (Gwida et al., 2010).

The diagnosis of brucellosis is usually performed by a combination of serological and molecular methods. Definitive diagnosis is usually carried out through isolation and identification of the causative organism, but drawback is that it is time-consuming, must be performed by highly skilled personnel, and is hazardous. For these reasons, serological tests like RBPT and serum agglutination test are normally preferred. For confirmation of findings of RBPT and SAT, ELISA based tests are used (Gul and Khan, 2007; Poester et al., 2010). In Pakistan, the prevalence of brucellosis has been reported to vary from 0 to 32.5% and work has been carried out on the seroprevalence of brucellosis in almost all domestic species like cattle and buffaloes (Asif et al., 2009), sheep and goats (Ghani et al., 1995; Iqbal et al., 2013), camel (Nasrin et al., 1998), horses (Gul et al., 2013) and humans (Mukhtar and Kokab, 2008; Asif et al.,