Serum small extracellular vesicles proteome of tuberculosis patients demonstrated deregulated immune response
PROTEOMICS–Clinical Applications, 2020•Wiley Online Library
Purpose Detailed understanding of host pathogen interaction in tuberculosis is an important
avenue for identifying novel therapeutic targets. Small extracellular vesicles (EVs) like
exosomes that are rich in proteins, nucleic acids and lipids, act as messengers and may
show altered composition in disease conditions. Experimental design In this case control
study, small EVs are isolated from serum of 58 subjects (all male, 33 (15–70) in years)
including drug naïve active tuberculosis (ATB: n= 22), non‐tuberculosis (NTB: n= 18), and …
avenue for identifying novel therapeutic targets. Small extracellular vesicles (EVs) like
exosomes that are rich in proteins, nucleic acids and lipids, act as messengers and may
show altered composition in disease conditions. Experimental design In this case control
study, small EVs are isolated from serum of 58 subjects (all male, 33 (15–70) in years)
including drug naïve active tuberculosis (ATB: n= 22), non‐tuberculosis (NTB: n= 18), and …
Purpose
Detailed understanding of host pathogen interaction in tuberculosis is an important avenue for identifying novel therapeutic targets. Small extracellular vesicles (EVs) like exosomes that are rich in proteins, nucleic acids and lipids, act as messengers and may show altered composition in disease conditions.
Experimental design
In this case control study, small EVs are isolated from serum of 58 subjects (all male, 33 (15–70) in years) including drug naïve active tuberculosis (ATB: n = 22), non‐tuberculosis (NTB: n = 18), and healthy subjects (n = 18). Serum small EVs proteome analysis is carried out using isobaric tag for relative and absolute quantification (iTRAQ) experiments and an independent sample (n = 36) is used for validation.
Results
A set of 132 and 68 proteins are identified in iTRAQ‐I (ATB/Healthy) and iTRAQ‐II (ATB/NTB) experiments, respectively. Four proteins (KYAT3, SERPINA1, HP, and APOC3) show deregulation (log2‐fold change > ±0.48, p < 0.05) in ATB with respect to healthy controls and Western blot data corroborated mass spectrometry findings.
Conclusions and clinical relevance
These important proteins, involved in neutrophil degranulation, plasma heme scavenging, kynurenine, and lipid metabolism, show deregulation in ATB patients. Identification of such a protein panel in circulating small EVs besides providing novel insights into their role in tuberculosis may prove to be useful targets to develop host‐directed therapeutic intervention.
Wiley Online Library以上显示的是最相近的搜索结果。 查看全部搜索结果