Comparative studies of gene regulation suggest an important role for natural selection in
shaping gene expression patterns within and between species. Most of these studies,
however, estimated gene expression levels using microarray probes designed to hybridize
to only a small proportion of each gene. Here, we used recently developed RNA sequencing
protocols, which sidestep this limitation, to assess intra-and interspecies variation in gene
regulatory processes in considerably more detail than was previously possible. Specifically …

Comparative studies of gene regulation suggest an important role for natural selection in
shaping gene expression patterns within and between species. Most of these studies,
however, estimated gene expression levels using microarray probes designed to hybridize
to only a small proportion of each gene. Here, we used recently developed RNA sequencing
protocols, which sidestep this limitation, to assess intra-and interspecies variation in gene
regulatory processes in considerably more detail than was previously possible. Specifically …