Two-photon-excited fluorescence lifetime imaging microscopy (FLIM) is a chemically specific
3-D sensing modality providing valuable information about the microstructure, composition
and function of a sample. However, a more widespread application of this technique is
hindered by the need for a sophisticated ultra-short pulse laser source and by speed
limitations of current FLIM detection systems. To overcome these limitations, we combined a
robust sub-nanosecond fiber laser as the excitation source with high analog bandwidth …

Newly developed microscopy methods have the goal to give researches in bio-molecular
science a better understanding of processes ongoing on a cellular level. Especially two-
photon excited fluorescence (TPEF) microscopy is a readily applied and widespread
modality. Compared to one photon fluorescence imaging, it is possible to image not only the
surface but also deeper lying structures. Together with fluorescence lifetime imaging (FLIM),
which provides information on the chemical composition of a specimen, deeper insights on a …