Soil management practices can have negative or positive effects on soil quality. Our objective was to assess the effect of long‐term agricultural practices by evaluating selected soil physical and chemical properties. Soil samples were collected from two depths (0 to 15 and 15 to 30 cm) within a native pasture and an adjacent agricultural field that was being used for three different crop rotations. Soil quality was quantified using aggregate stability, bulk density, soil texture and available water content as physical properties and pH, electrical conductivity, organic matter and available phosphorus as chemical properties. The farmland soils were functioning at 71 and 70 per cent of their full potential at the 0‐ to 15‐ and 15 to 30‐cm‐depth increments, respectively, whereas those from the pasture were functioning at 73 and 69 per cent, respectively. The assessment showed substantial loss in soil organic carbon following 50 years of farmland cultivation. Tillage and fertilizer applications were presumably the primary reasons for weaker spatial dependence within farmland at the 0‐ to 15‐cm depth. Grazing was postulated as the main reason for weaker spatial dependence within the pasture soils at the 15‐ to 30‐cm depth. Overall, we conclude that 50 years of cultivation has not caused soil quality to decline to a point that threatens sustainability of the agricultural fields. Copyright © 2011 John Wiley & Sons, Ltd.