The first successful attempt to generate genetically modified plants expressing a transgene
was preformed via T-DNA-based gene transfer employing Agrobacterium tumefaciens-
mediated genetic transformation. Limitations over infectivity and in vitro tissue culture led to
the development of other DNA delivery systems, such as the biolistic method. Herein, we
developed a new one-step protocol for transgenic soybean recovery by combining the two
different transformation methods. This protocol comprises the following steps: agrobacterial …

MELO, BP de| LOURENCO-TESSUTTI, IT| MORGANTE, CV| SANTOS, NC| PINHEIRO, LB|
LINS, CB de J.| SILVA, MCM| MACEDO, LLP| FONTES, EPB| GROSSI-DE-SA, MF| BRUNO
PAES DE MELO, UFV; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; CAROLINA
VIANNA MORGANTE, CPATSA; NAIARA CORDEIRO SANTOS; LUANNA BEZERRA
PINHEIRO, UCB; CAMILA BARROZO DE JESUS LINS; MARIA CRISTINA MATTAR DA
SILVA, Cenargen; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; ELIZABETH …