Spectral characteristics of the bryophyte carpet and mat subformation on Nelson Island (South Shetlands Islands) and Galindez Island (Argentine Islands, Graham Coast) were analyzed using spectral reflectance characteristics. A set of 9 specific reflectance indices were calculated and compared between two locations for the same type of moss vegetation formed by Sanionia georgicouncinata and Warnstorfia spp. The Normalized Difference Vegetation Index (NDVI) is efficient in discriminating between the two contrasting color/ecological moss community classes, ie such as less vigorous or dead and vigorous. However, NDVI is not sufficiently sensitive to discriminate intermediate vitality states. Presented data also demonstrates that complementary application of two indices, NDVI and Photochemical Reflectance Index (PRI), can be promising for followup studies focused on the determination of the color differences attributed to ecolophysiological state of a moss community. With the same values of NDVI, bryophyte carpet and mat subformation on Galindez Island are characterized by higher values of the OSAVI, which can be used as an indicator for further monitoring.