Sexed sperm in dogs is of interest because of being polytocous, and as a result, the greatest
number of offspring of the same sex can improve the market, although few studies assessing
sperm sexing have been performed in this species. The present study, therefore, was
conducted to evaluate the effects on sperm quality and the effectiveness of three
discontinuous density gradients to separate dog sperm containing X and Y chromosomes.
Thirty ejaculates from ten adult dogs were collected by digital manipulation of the penis …