Spin-label saturation-recovery EPR at W-band: applications to eye lens lipid membranes
Saturation-recovery (SR) EPR at W-band (94GHz) to obtain profiles of the membrane fluidity
and profiles of the oxygen transport parameter is demonstrated for lens lipid membranes
using phosphatidylcholine (n-PC), stearic acid (n-SASL), and cholesterol analog (ASL and
CSL) spin labels, and compared with results obtained in parallel experiments at X-band (9.4
GHz). Membranes were derived from the total lipids extracted from 2-year-old porcine lens
cortex and nucleus. Two findings are especially significant. First, measurements of the spin …
and profiles of the oxygen transport parameter is demonstrated for lens lipid membranes
using phosphatidylcholine (n-PC), stearic acid (n-SASL), and cholesterol analog (ASL and
CSL) spin labels, and compared with results obtained in parallel experiments at X-band (9.4
GHz). Membranes were derived from the total lipids extracted from 2-year-old porcine lens
cortex and nucleus. Two findings are especially significant. First, measurements of the spin …
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