In recent years there is a spurt in the interest regarding survival of Ayurvedic forms of medication. In the global perspective, there is a shift towards the use of medicine of herbal origin, as the dangers and the shortcoming of modern medicine have started getting more apparent, majority of Ayurvedic formulation are prepared from herbs. It is the cardinal responsibility of the regulatory authorities to ensure that the consumers get the medication, which guaranteed the purity, safety, potency and efficacy. As a result of this Standardization arise for maintaining a good coordination among the quality of raw herb material, in process materials and in final product. Present study was carried out to standardize Zymodyne syrup (ZS)-A Poly Herbal Formulation containing 17 herb materials in form of water extract. Standardization of ZS included various physicochemical parameters viz. Moisture determination, pH determination, Water & Alcohol soluble extractive value, Ash values which complied the standard limits. Phytochemical investigation showed the presence of various constituents those might be responsible for the therapeutic activity of Zymodyne syrup. The values of Heavy metal and Microbial analysis for ZS were also complied the standard limit.