Stimulatory effect of luteinizing hormone, insulin-like growth factor-1, and epidermal growth factor on vascular endothelial growth factor production in cultured bubaline …

VS Chouhan, SS Dangi, V Babitha, MR Verma, S Bag… - Theriogenology, 2015 - Elsevier
The purpose of this study was to evaluate the temporal (24, 48, and 72 hours) and dose-
dependent (0, 5, 10, and 100 ng/mL of LH, insulin-like growth factor 1 [IGF-1], and EGF) in
vitro expression and secretion patterns of vascular endothelial growth factor (VEGF) in luteal
cell culture during different stages of estrous cycle in water buffaloes. Corpus luteum
samples from ovaries of early luteal phase (ELP; Days 1–4), midluteal phase (Days 5–10),
and late luteal phase (Days 11–16) were collected from a local slaughterhouse. The …

Stimulatory effect of luteinizing hormone, insulin-like growth factor-1, and epidermal growth factor on progesterone secretion and viability of cultured bubaline luteal …

VS Chouhan, SS Dangi, B Vazhoor, VP Yadav… - Theriogenology, 2014 - Elsevier
Abstract We evaluated the temporal (24, 48 and 72 hours) and dose-dependent (5, 10, and
100 ng/mL of LH, IGF-1, and EGF, respectively) production and secretion of progesterone
(P4) in cultured luteal cells from different stages of estrous cycle as well as the expression of
steroidogenic acute regulatory protein (STARD1), cytochrome P450 cholesterol side-chain
cleavage (CYP11A1), and 3β-hydroxysteroid dehydrogenase (HSD3B), anti-apoptotic gene
PCNA, and pro-apoptotic gene BAX in luteal cells of mid-luteal phase in buffalo. Samples …
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