Strategies for performing dynamic gene perturbation experiments in flowers
DS Ó'Maoiléidigh, E Graciet, F Wellmer - Bio-protocol, 2016 - bio-protocol.org
Dissecting the gene regulatory networks (GRNs) underlying developmental processes is a
central goal in biology. The characterization of the GRNs underlying flower development has
received considerable attention, however, novel approaches are required to reveal temporal
and spatial aspects of these GRNs. Here, we provide an overview of the options available to
perform dynamic gene perturbations to identify downstream response genes at specific
stages of development in the flowers of Arabidopsis thaliana.
central goal in biology. The characterization of the GRNs underlying flower development has
received considerable attention, however, novel approaches are required to reveal temporal
and spatial aspects of these GRNs. Here, we provide an overview of the options available to
perform dynamic gene perturbations to identify downstream response genes at specific
stages of development in the flowers of Arabidopsis thaliana.
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