Using morphological techniques, we characterized the developmental reorganization that
takes place during the first weeks after explantation in area CA1 of organotypic hippocampal
cultures maintained at the interface between medium and a CO 2-enriched atmosphere.
Pyramidal neurones redistributed from a vertical into an horizontal cell layer in the middle of
a three-dimensional culture, with apical dendrites running above the pyramidal layer. Glial
cells redistributed into a thin layer at the bottom of the culture, forming an interface between …