To investigate the growth situation of bone marrow stroma stem cells (BMSCs) cell-sheet and endothelial progenitor cells (EPCs) on demineralized bone matrix (DBM) and explore the effect of DBM/BMSCs cell-sheet and DBM/EPCs complex on the construction of functional and vascularized tissue-engineered bone in mongrel. BMSC cell-sheet was prepared with cell-sheet engineering approach, EPCs were isolated from canine bone marrow and DBM was prepared from homologous bone. BMSCs cell-sheet and EPCs were inoculated on DBM respectively and observed by inverted phase contrast microscope and scanning electron microscope. The porosity of DBM was measured. The DBM/BMSCs cell-sheet and DBM/EPCs complex were successfully constructed. The BMSCs cell-sheet and EPCs adhered to DBM well and grew rapidly. The BMSCs cell-sheet and EPCs have good biocompatibility with DBM. The complex of DBM/BMSCs cell-sheet, DBM/EPCs could provide good conditions to acquire functional and vascularized tissue-engineered bone.