Green fluorescent protein (GFP) is a light-emitting protein that does not require a prosthetic
group for its fluorescent activity. As such, GFP has become indispensable as a molecular
tool in molecular biology. Nonetheless, there has been no subatomic elucidation of the GFP
structure owing to the structural polymorphism around the chromophore. Here, subatomic
resolution X-ray structures of GFP without the structural polymorphism are reported. The
positions of H atoms, hydrogen-bonding network patterns and accurate geometric …