Subunit mass analysis for monitoring antibody oxidation
I Sokolowska, J Mo, J Dong, MJ Lewis, P Hu - MAbs, 2017 - Taylor & Francis
Methionine oxidation is a common posttranslational modification (PTM) of monoclonal
antibodies (mAbs). Oxidation can reduce the in-vivo half-life, efficacy and stability of the
product. Peptide mapping is commonly used to monitor the levels of oxidation, but this is a
relatively time-consuming method. A high-throughput, automated subunit mass analysis
method was developed to monitor antibody methionine oxidation. In this method, samples
were treated with IdeS, EndoS and dithiothreitol to generate three individual IgG subunits …
antibodies (mAbs). Oxidation can reduce the in-vivo half-life, efficacy and stability of the
product. Peptide mapping is commonly used to monitor the levels of oxidation, but this is a
relatively time-consuming method. A high-throughput, automated subunit mass analysis
method was developed to monitor antibody methionine oxidation. In this method, samples
were treated with IdeS, EndoS and dithiothreitol to generate three individual IgG subunits …
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