The present study summarizes the valorization of date flour by the production of lipopeptide biosurfactant (BioS) by Bacillus subtilis ZNI5 (MW091416). A Taguchi design permitted the formulation of a medium composed only of 6% date flour and 0.5% yeast extract within 2 days of incubation at 150 rpm with a maximal surface tension (ST) reduction of about 27.8 mN/m. The characterization of the lipopeptide shows a CMC value of about 400 mg/L with a minimal ST of 30 mN/m and an ability to disperse oil to about 80 mm at 800 mg/L. Having reduced phytotoxicity, the ZNI5 BioS and ZNI5 strain were assayed for Copper and Cobalt chelation and biosorption. The improvement of the germination index of radish seeds irrigated by the treated contaminated water showed the great potential application of ZNI5 lipopeptide in the bioremediation of heavy metals.