Systems biology approach identifies the kinase Csnk1a1 as a regulator of the DNA damage response in embryonic stem cells
J Carreras Puigvert, L von Stechow, R Siddappa… - Science …, 2013 - science.org
In pluripotent stem cells, DNA damage triggers loss of pluripotency and apoptosis as a
safeguard to exclude damaged DNA from the lineage. An intricate DNA damage response
(DDR) signaling network ensures that the response is proportional to the severity of the
damage. We combined an RNA interference screen targeting all kinases, phosphatases,
and transcription factors with global transcriptomics and phosphoproteomics to map the
DDR in mouse embryonic stem cells treated with the DNA cross-linker cisplatin. Networks …
safeguard to exclude damaged DNA from the lineage. An intricate DNA damage response
(DDR) signaling network ensures that the response is proportional to the severity of the
damage. We combined an RNA interference screen targeting all kinases, phosphatases,
and transcription factors with global transcriptomics and phosphoproteomics to map the
DDR in mouse embryonic stem cells treated with the DNA cross-linker cisplatin. Networks …
[PDF][PDF] Systems Biology Approach Identifies the Kinase Csnk1a1 as a Regulator
JHN Meerman, LHF Mullenders, B van de Water, E HJ - researchgate.net
In pluripotent stem cells, DNA damage triggers loss of pluripotency and apoptosis as a
safeguard to exclude damaged DNA from the lineage. An intricate DNA damage response
(DDR) signaling network ensures that the response is proportional to the severity of the
damage. We combined an RNA interference screen targeting all kinases, phosphatases,
and transcription factors with global transcriptomics and phosphoproteomics to map the
DDR in mouse embryonic stem cells treated with the DNA cross-linker cisplatin. Networks …
safeguard to exclude damaged DNA from the lineage. An intricate DNA damage response
(DDR) signaling network ensures that the response is proportional to the severity of the
damage. We combined an RNA interference screen targeting all kinases, phosphatases,
and transcription factors with global transcriptomics and phosphoproteomics to map the
DDR in mouse embryonic stem cells treated with the DNA cross-linker cisplatin. Networks …