Passion fruit seeds are rich in fat. Oil of the seeds can be obtained through mechanical extraction. Moisture content is one of the factors, determine the result extraction of the seeds. This study investigates the effects of moisture content towards oil produced by pressing tests using a screw press. The seeds were conditioned at 5%, 7%, and 11% moisture content (wet basis); pressing temperature, 62 C. Chemical analyses ie refractive index and peroxide value, are conducted to determine the quality of oil through treatment of moisture content that produces the most oil. The result shows that oil yield increases with the increasing moisture content of the seeds. The highest amount of oil (12.32%) produced by 11% moisture content. The 7% of moisture content showed the highest unsaturated fatty acid, which was 70.928±17.430%(linoleic acid) and 17.947±6.944%(oleic acid). These results indicated that passion fruit seeds oil has possibility as edible vegetable oils.