The N-terminal domain of 5-lipoxygenase binds calcium and mediates calcium stimulation of enzyme activity
Human 5-lipoxygenase (5-LO) is a key enzyme in the conversion of arachidonic acid into
leukotrienes and lipoxins, mediators and modulators of inflammation. In this study, we
localized a stimulatory Ca 2+-binding site to the N-terminal region of the enzyme. Thus, in a
45 Ca 2+ overlay assay, the N-terminal 128 amino acids of recombinant human 5-LO (fused
to glutathione S-transferase) bound radioactive calcium to about the same extent as intact 5-
LO. The glutathioneS-transferase fusion protein of the C-terminal part of 5-LO (amino acids …
leukotrienes and lipoxins, mediators and modulators of inflammation. In this study, we
localized a stimulatory Ca 2+-binding site to the N-terminal region of the enzyme. Thus, in a
45 Ca 2+ overlay assay, the N-terminal 128 amino acids of recombinant human 5-LO (fused
to glutathione S-transferase) bound radioactive calcium to about the same extent as intact 5-
LO. The glutathioneS-transferase fusion protein of the C-terminal part of 5-LO (amino acids …