This paper presents data investigating the effect of fatty acid methyl ester (FAME) both with and without additives on B5 cold flow performance. Cold chamber chassis dynamometer (CCCD) testing has been used to evaluate fuel performance in a light duty European truck. The effect of the Infineum biodiesel cold-flow improver (BDFI) was to improve cold start and operability performance. The no-harms of high treat rates of Infineum BDFI have also been demonstrated. Two passenger cars running on 100% rapeseed methyl ester (RME), both untreated and treated with 10,000 ppm BDFI, have been used to accumulate 10,000 km to assess the impact of high additive treat rates on driveability, emissions and performance. The BDFI had no harms effect on any measured performance parameter.