Delayed onset muscle soreness (DOMS) is the sensation of stiffness and pain in the muscles that occurs 24-48 hours after unaccustomed or strenuous EXERCISE. The aim of this study was to examine and compare the effect of VIBRATION training (VT) on Delayed onset muscle soreness. Twenty healthy non-athletic volunteers (Female, age 20-30 years, body mass 23.27±0.82 kg) were randomly assigned to VT (n= 10) and non-VT (n= 10) groups. A vibrator was used to apply 50-30 Hz of VIBRATION on the left quadriceps and calf muscles in the VT group, while no VIBRATION was applied for the non-VT group. Then, both groups ran downhill on a-5 treadmill at a speed of 5-7.5 km/hour (increasing speed). Twenty-four-hours before and after the VIBRATION training, serum CREATINE KINASE levels, pressure pain threshold and the rate of edema were measured. Compared with the non VT group, the VT group showed a significant decrease in pressure pain threshold at 15 cm of patellar point (P= 0.042) and calf muscle (P= 0.041), there were no significant differences between groups in the pressure pain threshold at 5 cm (P= 0.509) and 10 cm (P= 0.152) points. There was no significant change in the edema rate at the Gastrocnemius muscle (P= 0.420) and midpoint of quadriceps muscle (P= 0.380) in VT and non-VT groups. Despite the increase in CREATINE KINASE enzymes in the two groups, there was no statistically significant difference between the groups (P= 0.173). The results showed that using the VIBRATION training can affect delayed onset muscle soreness (DOMS) reduction between non-VT and VT groups. Further studies with larger samples are needed to determine the VIBRATION training positive and negative effects.