The present research aims to examine the effect of aerobic exercise with medium and high intensity on the gene expression of Bax and Bcl-2 markers in rat myocard after ischemic-reperfusion by isoprenaline. 26 male Wistar rats were used weighing approximately 225.25±25.25 g with two to three months old; Then pilot study with stroke group (n = 7) and control Group (n = 7) was knock to examine of isopernalin induced-infarct and the infarct of left ventricle were confirmed in isopernalin into normal groups used Masson-trichrom teqnique. Then rats were divided into three groups to Measure gene expression: control (n=4), aerobic exercise with middle (n=4) and many intensity (n=4). The rats in research group were introduced to treadmill for a week, then the rats were exposed medium and high intensity aerobic training with frequency of three times weekly for one month. After two days of rest, rats were injected with isoprenaline within 24 hours; but control group were injected only with isopernaline in the end of one month. The formula 2-ΔΔct were used to measure the gene expression of BAX and BCL2, and independent t-test was used to compare the two groups of stroke and control in the pilot study and ANOVA were used to compare the groups.The results of study showed that aerobic exercise with medium and high intensity could reduce and increase the gene expression of BAX and BCL2, but these changes were not significant. Therefore, it seems that this intensity and period of training can not active anti-apoptotic routes of exercise to minimize the effects of stroke.