Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia caused by defects in insulin secretion, insulin action, or both. Hyperglycemia in chronic DM can trigger various complications, both macrovascular and microvascular, one of which is diabetic nephropathy. Diabetic nephropathy or diabetic kidney disease (PGD) is a complication that often occurs in diabetics. This study aims to determine the content of secondary metabolites in lime leaf extract on reducing blood glucose levels, pancreatic histopathology and diabetic nephropathy in male white rats (Rattusnorvegicus) induced by streptozotocin. This study used 30 male white rats which were divided into 6 groups, namely group I as normal control, group II as positive control (glibenclamide), group III as negative control (Na-CMC 0.5%), group IV, V and VI as control. the test group was given ethanol extract of lime leaves with doses of 150mg/kgBW, 250mg/kgBB, 350mg/kgBW, respectively. Data were analyzed using a one way ANOVA test at a 95% confidence level, followed by a post hoc LSD test and using a nonparametric test. Showed that the ethanol extract of lime leaves (citrus aurantifolia (christm.) swingle) contained 1.419% w/w alkaloids, 0.008% w/w tannins, 0.687% w/w flavonoids, and 15.805% w/w saponins. b and can reduce glucose levels at a dose of 350mg/kg BW and can regenerate pancreatic tissue at a dose of 350mg/kg BW damage 0.8 and repair renal tubular injury at a dose of 250mg/kg BW damage 1.2. this study contains secondary metabolites and can reduce blood glucose levels in rats and can regenerate pancreatic tissue and kidney tubular injury.