The fluorescent pH-indicator BCECF: An inhibitor of the vacuolar ATPase from Chenopodium rubrum
W Pfeiffer, M Höftberger - Journal of plant physiology, 2000 - Elsevier
Selective vacuolar accumulation of the fluorescent pH-indicator 2′, 7′-bis (2-
Carboxyethyl)-5 (6)-carboxyfluorescein (BCECF) may allow to study vacuolar acidification in
vivo (Brauer et ai., 1995), which in plant cells is driven by two distinct proton pumps: the V-
ATPase (EC 3.6. 1.3) and the V-PPase (EC 3.6. 1.1). The use of BCECF may help to
investigate these proton pumps in vivo. A necessary precondition for such a study is that
both enzymes are not affected by the indicator. This was investigated using tonoplast …
Carboxyethyl)-5 (6)-carboxyfluorescein (BCECF) may allow to study vacuolar acidification in
vivo (Brauer et ai., 1995), which in plant cells is driven by two distinct proton pumps: the V-
ATPase (EC 3.6. 1.3) and the V-PPase (EC 3.6. 1.1). The use of BCECF may help to
investigate these proton pumps in vivo. A necessary precondition for such a study is that
both enzymes are not affected by the indicator. This was investigated using tonoplast …
以上显示的是最相近的搜索结果。 查看全部搜索结果