Metabolic fluxes may be regulated “hierarchically,” eg, by changes of gene expression that
adjust enzyme capacities (V max) and/or “metabolically” by interactions of enzymes with
substrates, products, or allosteric effectors. In the present study, a method is developed to
dissect the hierarchical regulation into contributions by transcription, translation, protein
degradation, and posttranslational modification. The method was applied to the regulation of
fluxes through individual glycolytic enzymes when the yeast Saccharomyces cerevisiae was …