[PDF][PDF] The influence of respiration and ATP hydrolysis on the proton-electrochemical gradient across the inner membrane of rat-liver mitochondria as determined by …
DG Nicholls - European Journal of Biochemistry, 1974 - researchgate.net
European Journal of Biochemistry, 1974•researchgate.net
MATERIALS AND METHODS Mitochondria were prepared from rat liver by differential
centrifugation in 0.25 M sucrose. Oxygen uptake was determined by a Clark-type oxygen
electrode in a perspex chamber of 0.9 ml capacity. All experiments were performed at 23" C,
and the initial pH of the incubation media was 7.2. Protein was determined by the biuret
method. Oligomycin, rotenone, sodium succinate, sodium~~-3-hydroxybutyrate, ATP and
ADP were obtained from Sigma Chemical Co.; valinomycin from Calbiochem, and all …
centrifugation in 0.25 M sucrose. Oxygen uptake was determined by a Clark-type oxygen
electrode in a perspex chamber of 0.9 ml capacity. All experiments were performed at 23" C,
and the initial pH of the incubation media was 7.2. Protein was determined by the biuret
method. Oligomycin, rotenone, sodium succinate, sodium~~-3-hydroxybutyrate, ATP and
ADP were obtained from Sigma Chemical Co.; valinomycin from Calbiochem, and all …
MATERIALS AND METHODS
Mitochondria were prepared from rat liver by differential centrifugation in 0.25 M sucrose. Oxygen uptake was determined by a Clark-type oxygen electrode in a perspex chamber of 0.9 ml capacity. All experiments were performed at 23" C, and the initial pH of the incubation media was 7.2. Protein was determined by the biuret method. Oligomycin, rotenone, sodium succinate, sodium~~-3-hydroxybutyrate, ATP and ADP were obtained from Sigma Chemical Co.; valinomycin from Calbiochem, and all isotopes from the Radiochemical Centre (Amersham, England). FCCP was a gift from Dr P. G. Heytler. All other reagents were of the highest purity commercially available.
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