… platelet donors) have not been performed. However, we found that use of platelet-derived
microparticles as a platelet … donor platelets were handled identically in both assays 34. …

… ; however, some of these tools, such as the widely used separation-based HPLC technique
and the current gold-standard release assay based on [ 14 C]-serotonin radiolabeling, …

… test for HIT utilizing washed donor platelets, called the platelet serotonin-release assay (SRA),
was developed at the McMaster University Platelet Immunology Laboratory.10 By utilizing …

… arc required for platelets to release serotonin (Humphrey & … for serotonin rclcasc, acid eluates
of platelets and 7s gamma … used in the serotonin release test. Immunoglobulin conteiit of …

… Finally, this study suggests that serotonin release could be measured by non‐radioactive
SRA, especially HPLC, to assess an HIT with good sensitivity and specificity. Each department …

… Figure 1: Models for irreversible and reversible changes in platelet serotonin (5-…
Serotonin released from dense granules might be important in downstream events during …

… of platelet serotonin by HPLC; (iv) an indirect assay that … 2+ or ATP concentration and
serotonin inside the granules, … , can replace the radioactive serotonin release assay for the …

… platelets in some manner which enhances serotonin release. In an attempt to increase
serotonin release … Despite the fact that thrombin is able to release serotonin from the platelets (8, …

… -Serotonin release assay (14C-SRA) from platelets is considered to be the most sensitive test
for … (EIA) to determine the release of serotonin from platelets in the presence of heparin and …

… For the determination of total serotonin in platelets and plasma, the PRP … platelet serotonin,
plasma and platelets were separated by centrifugation at 1000 g for 15 min and the platelet …